Endo-parasites and helpful organisms
Almost two years ago, Julius Lukes sat down to a meal of raw fish riddled with tapeworm eggs. “I did not enjoy it,” he recalls. Happily, it took just a second or two: “You put it on your spoon and shovel it down.”
Today, his intestines are home to three tapeworms with a total length of about 20 metres – all because Mr. Lukes, a senior fellow at the Canadian Institute for Advanced Research (CIFAR) and a professor at the University of South Bohemia in the Czech Republic, belongs to a small but growing group of scientists who maintain that some parasites may actually be good for us. In other words, the dictionary isn’t completely accurate when it says that a parasite “obtains nourishment” from its host, “which does not benefit from the association, and is often harmed by it.”
The researchers are exploring whether parasites could be used to treat a raft of autoimmune disorders, such as inflammatory bowel disease, multiple sclerosis and arthritis – chronic and often painful conditions in which the body’s immune system produces antibodies that attack its own tissues. Studies have repeatedly shown that the incidence of such disorders is highest in the developed world, where people live in relatively sterile environments.
Inflammatory bowel disease – Crohn’s disease and ulcerative colitis – affects about a quarter-million Canadians. An estimated 100,000 in this country have multiple sclerosis, one of the highest rates in the world. And arthritis affects no fewer than 4.5 million of us. There is no cure for any of these illnesses – only treatments that provide inconsistent relief.
But what if there is a cure, and we’ve been too squeamish to take it seriously? Scientists are beginning to investigate a theory that parasites can prime the immune system so that, when faced with a minor insult such as gluten or cheese, for example, it does not overreact and cause a serious disorder.
In a recent review in Trends in Parasitology, Mr. Lukes and his colleagues go so far as to call some parasites “old friends” who have evolved along with us over millions of years. Today’s overly hygienic lifestyle, however, has reduced our contact with these friends, perhaps sparking the rising rates of autoimmune disorders.
Not that every one gets a pass. “There is no good parasite in human blood or in the brain,” says Mr. Lukes, who is also a researcher at the Czech Academy of Sciences. “The argument is for intestinal parasites only.”
Experiments with rodents already suggest that infection with worm-like intestinal parasites called helminths can provide relief from autoimmune disorders. As well as his one-man study of tapeworms, Mr. Lukes plans to add to the helminth research with a rodent study of his own. He is collaborating with University of British Columbia microbial ecologist Laura Wegener Parfrey on a CIFAR-sponsored study of two parasites: a species of tapeworm called hymenolepis and a single-celled parasite called a blastocystis. Working with rats suffering from a form of inflammatory bowel disease, they will see what impact both parasites have on the illness, and hope to have results next fall.
Ms. Wegener Parfrey’s previous research suggests parasites are a normal part of a healthy gut. In a study published in June in Frontiers in Microbiology, she found that a greater variety of parasites live in the guts of healthy people in remote areas of Malawi than in those of North Americans. “The ubiquity of parasites in healthy people in developing countries and high abundance even in westernized countries shaped my thinking that parasites are likely good, at least some of the time,” she says.
According to Ms. Wegener Parfrey, the critical time for infection is probably in childhood, when our immune system is still developing. But small clinical trials in Europe and North America involving adults suggest that infection with worms in particular can reduce symptoms of some autoimmune diseases. Biopharmaceutical companies Ovamed GmbH in Germany and Coronado Biosciences in Massachusetts are conducting trials involving T. suis, a pig whipworm that sometimes infects humans and may be effective against inflammatory bowel disease, multiple sclerosis and arthritis.
T. suis is a good candidate for therapeutic infection because it can’t complete its life cycle in humans: To survive, it must find its way into a pig at some point, meaning it can’t stick around in the human gut long enough to cause serious problems. Other good candidates include parasites that don’t migrate to the blood or brain, and those that aren’t infectious when excreted from the body.
Mr. Lukes’s tapeworms – a species called Diphyllobothrium latum – fit the bill on all counts. He does not suffer from an autoimmune disorder; the point of his experiment is to see whether the worms can do him any harm. And so far, so good, he says – despite the fact that his tenants are not all that much shorter than the newly named Dreadnoughtus, the biggest dinosaur. He has experienced none of the diarrhea or vomiting typically considered symptoms of infestation.
Medical textbooks suggest he should be suffering a vitamin B12 deficiency by now, but tests show that he is healthy. “I feel wonderful,” he says.
As a guinea pig, Mr. Lukes is not alone. Last year, James Logan, a parasitologist at the London School of Hygiene and Tropical Medicine, infected himself with hookworms, providing detailed online video documentation of his body’s reaction. Mr. Logan did not feel wonderful. He suffered stabbing abdominal pains that kept him up at night. On the other hand, he found relief from a long-standing allergy to bread products – an immune-system overreaction to something normally harmless. After two months, he swallowed a pill to get rid of his parasite along with his new-found ability to eat bread.
Mr. Lukes could do the same for his tapeworms, but says he won’t. In fact, last month he tried to infect himself with giardia, a microscopic parasite known among North American backpackers as the cause of “beaver fever.” It is most often transmitted through contaminated water and is widely believed to cause violent diarrhea, nausea and cramping that can last weeks without treatment. “Everybody freaks out about giardia,” says Mr. Lukes, whose first dose didn’t take – although he plans to down another one soon. “But in most cases, it doesn’t do anything. So that’s my testable hypothesis. That can be a follow-up story in a year.”
Source: The Globe and Mail © Copyright 2014 The Globe and Mail Inc. (17/11/14)
Peptides naturally produced by parasitic intestinal worms could help treat auto-immune diseases such as multiple sclerosis and rheumatoid arthritis, say researchers at Monash University.
Peptides naturally produced by intestinal worms could help treat auto-immune diseases such as multiple sclerosis, rheumatoid arthritis, lupus and psoriasis. That is the finding of a Monash University study, published in the journal of the Federation of American Societies for Experimental Biology.
Professor Ray Norton, lead researcher at Monash Institute of Pharmaceutical Sciences, said the research was possible through the "wonders of the genome era". His team had been working on molecules from a sea anemone that can suppress the effects of auto-immune diseases.
Prof Norton said scientists in the USA had identified that parasitic worms had the capability to produce similar molecules.
"We went looking to see if these molecules really were in the worms," he said.
"Sure enough, these molecules can actually suppress the sorts of white blood cells that cause all the damage in auto-immune diseases."
The research could be seen as supporting the "hygiene hypothesis" that developed societies are too clean for their own good.
"There's no doubt that as our environment gets cleaner and we're exposed to fewer and fewer infections that allergies and, to a lesser extent, auto-immune diseases are on the rise," said Prof Norton.
However, Prof Norton said that while "it's a good correlation" the causal effect of the link is "very much up for debate."
In fact one of the key scientists in the Monash study is sceptical of the hypothesis.
"He accepts the link but he doesn't accept the current explanation."
The researchers hope that their work could lead to a pill that mimics the benefits of parasitic worm infection.
Professor Norton believes producing a pharmaceutical is preferable to infecting people with worms.
"There are people culturing worms on huge scales for clinical trials in the US.
"The question will still be is the worm infection just doing you good, or is it potentially causing some problems as well."
He said given the choice he would prefer to take a pill than be given intestinal worms.
"A pure pharmaceutical might actually be much cleaner and more effective."
Source: ABC Melbourne © 2014 ABC (12/08/14)
The pig whipworm could help treat people suffering from arthritis, multiple sclerosis, diabetes and autism.
Medical experts are excited at the unlikely new hope a parasitic worm found in pigs could offer across a suite of illnesses and ailments.
University of Melbourne has for the first time mapped the genes of the pig whipworm, which could lead to new drugs and treatments for auto-immune disorders.
The pig whipworm causes death in livestock, but it does not harm humans, and there is even evidence it can lessen symptoms in people with multiple sclerosis and inflammatory bowel disease.
In auto-immune disorders the immune system mistakenly attacks their body’s own tissues.
Already the pig whipworm is being used as medicine for treating inflammatory bowel diseases.
“It seems to affect the immune system by suppressing some of the inflammation,” the university’s Faculty of Veterinary Science Dr Aaron Jex said.
“The goal is to see if we can identify what it is doing in the body and how you could mimic that with a drug.”
Understanding the genetic make up of the parasite that infects animals will also help scientists better understand the human whipworm, which infects an estimated one billion people worldwide.
Dr Jex said people can become infected with the human form of the parasite after ingesting food or water with the eggs in it.
It can lead to dysentery, malnourishment and, in children in particular, it can lead to problems in physical and mental development.
The whipworm, which is between 2cm and 4cm in length, implants it’s front end in the intestinal wall and its body sticks out, like the handle of a whip.
“The long thin head is what it uses to feed in the intestinal wall,” Dr Jex said.
The adult worm secretes enzymes that breaks down cells in their host.
For the past two years Dr Jex has been sequencing the pig whipworm’s genome, working in collaboration with 11 institutions in six countries.
The paper, published in the journal Nature Genetics today, states that understanding the worm’s genetic make-up should enable the design of urgently needed drugs to treat the human form of the disease.
Dr Jex said the human whipworm is predominately found in south east Asia in rural or remote areas, particularly where there are inadequate sewerage systems.
There is one treatment that is currently being distributed to children in countries most at risk, but alternatives are needed to prevent drug resistance.
Source: Herald Sun Copyright News Ltd 2014 (16/06/14)